{"id":534,"date":"2022-10-12T08:21:18","date_gmt":"2022-10-12T08:21:18","guid":{"rendered":"https:\/\/mebicar.pl\/sklep\/methylene-blue-methylene-blue-purity-%e2%89%a5-99-99-1000mg\/"},"modified":"2026-08-13T18:24:26","modified_gmt":"2022-10-22T17:36:45","slug":"methylene-blue","status":"publish","type":"product","link":"https:\/\/mebicar.pl\/en\/sklep\/methylene-blue\/","title":{"rendered":"Methylene Blue 1 g \u2014 Bench Notes on Dye Content, Hydration and Clean Staining"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">What the bottle actually contains<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Methylthioninium chloride is a cationic thiazine dye, and the label carries more than one number worth reading.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Registry number 61-73-4, quoted on orders, in the register and on waste documentation.<\/li><li>Molecular formula C16H18ClN3S with a molecular weight of 319.85 for the anhydrous chloride salt.<\/li><li>Physical form: dark green to bronze crystalline powder that gives an intense blue aqueous solution.<\/li><li>High water solubility, which is why the dye is the default choice for aqueous staining baths.<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Why dye content is not chromatographic purity<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Dyes are traditionally specified by dye content, a figure that describes how much of the weighed mass is the coloured species itself.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>The remainder is typically water of hydration, buffer salts and isomeric or demethylated relatives of the main structure.<\/li><li>A lot at 82 percent dye content is not an adulterated lot; it is a normally specified one, and the figure is used in the calculation rather than ignored.<\/li><li>Grades certified for microscopy, for microbiology and for spectrophotometric work differ in exactly this table.<\/li><li>Any calculation of molar concentration has to use the dye content figure from the certificate, not the nominal formula weight alone.<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Hydration and weighing<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Commercial material is commonly supplied hydrated, so the mass on the balance and the mass of dye in the flask are different quantities.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Check whether the certificate refers to the anhydrous salt or to a hydrate before converting mass into concentration.<\/li><li>Weigh on paper or in a closed vessel; the powder is light, electrostatic and stains everything it touches.<\/li><li>Keep a dedicated spatula for this material, because trace carryover is visible for a long time afterwards.<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Bench work where the grade decides the outcome<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The compound serves several distinct roles, and each of them stresses a different line of the specification.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>As a simple stain and counterstain in microscopy, where optical grade and absence of insoluble particles matter most.<\/li><li>As a redox indicator, exploiting the reversible change between the blue oxidised form and the colourless reduced form.<\/li><li>As a probe of adsorbent surface area, where the dye content figure enters the calculation directly.<\/li><li>As a reference absorber in spectrophotometer checks, where lot to lot consistency is the point.<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Storage, spills and disposal<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Storage is undemanding; the clean-up discipline is not.<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>Keep the container closed and dry, away from strong oxidising agents and direct sunlight.<\/li><li>Prepare stock solutions in labelled amber or opaque bottles and filter them before spectrophotometric use.<\/li><li>Wipe spills immediately with a damp cloth; dry powder disperses and colours a wide area of bench and floor.<\/li><li>Collect dye waste separately, since coloured effluent is a visible and traceable discharge.<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">See also<\/h2>\n\n\n\n<ul class=\"powiazane-odczynniki wp-block-list\">\n<li><a href=\"\/sklep\/alpha-gpc\/\">Alpha GPC, purity \u2265 99% weight 1000mg net.<\/a><\/li>\n<li><a href=\"\/sklep\/noopept\/\">Noopept \/ \u041d\u041e\u041e\u041f\u0415\u041f\u0422. Syntetyczna substancja \u2265 99% 1000MG.<\/a><\/li>\n<li><a href=\"\/sklep\/idra-21-2\/\">IDRA-21, purity \u2265 99% 1000mg<\/a><\/li>\n<li><a href=\"\/sklep\/cannabidiol-2\/\">CBD \/ Cannabidiol \/ Cannabidiol, Krishals \u2265 99.85% 1000mg!<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">References<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Animal and in vitro studies indexed in PubMed; no human-use guidance is provided here.<\/p>\n\n\n\n<ul class=\"pismiennictwo wp-block-list\">\n<li>Wiklund L <em>Neuroprotection by Methylene Blue in Cerebral Global Ischemic Injury Induced Blood-Brain Barrier Disruption and Brain Pathology: A Review<\/em> 2016, CNS &amp; neurological disorders drug targets. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27633785\/\" rel=\"nofollow noopener\" target=\"_blank\">PMID 27633785<\/a><\/li>\n<li>Batista-Filho MM <em>Role of Methylene Blue in Trauma Neuroprotection and Neuropsychiatric Diseases<\/em> 2016, CNS &amp; neurological disorders drug targets. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27450870\/\" rel=\"nofollow noopener\" target=\"_blank\">PMID 27450870<\/a><\/li>\n<li>Rojas JC <em>Neurometabolic mechanisms for memory enhancement and neuroprotection of methylene blue<\/em> 2012, Progress in neurobiology. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22067440\/\" rel=\"nofollow noopener\" target=\"_blank\">PMID 22067440<\/a><\/li>\n<li>Tucker D <em>From Mitochondrial Function to Neuroprotection-an Emerging Role for Methylene Blue<\/em> 2018, Molecular neurobiology. <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28840449\/\" rel=\"nofollow noopener\" target=\"_blank\">PMID 28840449<\/a><\/li>\n<\/ul>\n\n\n\n<blockquote class=\"odczynnik-status\"><p>Product classification. It is not a substance for human use. The material is not a veterinary product. A high-purity laboratory product used in HPLC and GC-MS studies. Outside the category of medicinal and food products.<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>What the bottle actually contains Methylthioninium chloride is a cationic thiazine dye, and the label carries more than one number worth reading. Registry number 61-73-4, quoted on orders, in the register and on waste documentation. Molecular formula C16H18ClN3S with a molecular weight of 319.85 for the anhydrous chloride salt. Physical form: dark green to bronze [&hellip;]<\/p>\n","protected":false},"featured_media":511,"template":"","meta":[],"product_brand":[],"product_cat":[171],"product_tag":[185,162,184,186],"class_list":["post-534","product","type-product","status-publish","has-post-thumbnail","product_cat-synthetic-en","product_tag-cas-number-61-73-4-en","product_tag-methylene-blue-en","product_tag-methylene-blue-en-2","product_tag-methylthioninium-chloride","first","instock","shipping-taxable","purchasable","product-type-variable"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Methylene Blue 1 g \u2014 Dye Content and Hydration Notes<\/title>\n<meta name=\"description\" content=\"Methylene blue (methylthioninium chloride), CAS 61-73-4, C16H18ClN3S, mass 319.85: dye content, hydrate water, redox indicator work and batch records.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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